The enzyme concentration estimated by titration with okadaic acid agreed well with that calculated from the protein content and the molecular mass for type 2A phosphatase. Cells were cultured for 2 hours in the presence of a final concentration of 10nM Calyculin A and 500nM Okadaic acid. Okadaic acid was detected in concentrations as low as 0.063 ng/ml in aqueous solutions and 2 ng/g in mussel digestive glands. In higher concentrations Okadaic acid induced apoptosis (IC 50 = 0.5 mM). 1-4. Okadaic acid was found to induce concentration- and time-dependent lipolysis in rat fat cells in the absence of lipolytic hormones, but it. Is it possible to activate some kinase activity (it’s been published that okadaic acid can activate PKC activity) in. The molecular formula of okadaic acid, which is a derivative of a C 38 fatty acid. Okadaic acid at the lowest concentration used did not show any visible effect on mitosis. The demosponge Suberites domuncula contains the bioactive compound okadaic acid; concentrations of ca. 50 ng/g (ca. 60 nM) of wet tissue have been quantiï¬ed by LC/MS. Construct a standard curve by plotting the %B/B0 for each standard on the vertical linear (y) axis versus the corresponding Okadaic Acid concentration on the horizontal logarithmic. Cells were washed with ice-cold PBS and lysed in RIPA buffer.
Okadaic acid is a toxin that accumulates in bivalves and causes diarrheal shellfish poisoning. Quantitation of the ADAM derivative indicated that the concentration. Similar repression was obtained with low concentrations of. Okadaic acid was found to induce concentration- and time-dependent lipolysis in rat fat cells in the absence of lipolytic hormones, but it did not. For the phosphorylated myosin light-chain (PMLC) phosphatase activity of the enzyme, the concentration of okadaic acid required to obtain 50% inhibition (ID50) was about 1 nM. Okadaic acid completely inhibited the PB induction process in a concentration-dependent manner (IC 50, ?1.5?2 nM). The results presented here come from cells treated with OA at concentrations Abstract. Construct a standard curve by plotting the %B/B0 for each standard on the vertical linear (y) axis versus the corresponding Okadaic Acid concentration on the horizontal. Primary hepatocytes were cultured for 48 h and then exposeto increasing okadaic acid concentrations for 60 min before, and then continuously in the. Okadaic acid down-regulated the TNF receptor, induces the release of Ca 2+ from intracellular. Mitotic HeLa cells were treated with different concentrations of okadaic acid (OA), known to inhibit phosphatase 1 and 2A activities.
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Okadaic Acid Concentration